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Hyperspectral Luminescence Microscopy

Photon etc IMA Photoluminance Microscopy System

IMA™ Hyperspectral Microscopy Platform

IMA™ from Photon etc is an ultrafast and "all-in-one", customisable hyperspectral microscopy platform that provides both high spatial and high spectral resolution. The completely integrated system rapidly maps diffuse reflectance, transmittance, photoluminescence, electroluminescence and fluorescence in the VIS-NIR-SWIR spectral range. Based on high throughput global imaging filters, IMA™ is faster and more efficient than scanning spectrograph-based hyperspectral systems.

IMA enables complex material analysis such as the characterisation of solar cells and perovskite, the mapping of composition, stress, defects in materials, the monitoring of spectral information, the changes in the intensity of single emitters, wavelength shifts or spectral bandwidth variations. Imaging from 400 to 1620nm with a bandwidth of 3nm, the IMA is capable of measuring optoelectrical properties such as Voltage Open Circuit and External Quantum Efficiency and allows precise detection and characterisation of defects in materials which is ideal for the quality control of semiconductor devices (GaAs, SiC, CdTe, CIS, CIGS, etc.).

The spectral range covered by IMA is ideal for the spatial and spectral identification and measurement of fluorophores that emit in the second biological window. With the possible integration of a darkfield illumination module, it becomes an exceptional tool to detect the composition and the location of nanomaterials embedded in cells or the complex analysis of live, in-vitro and unstained biological samples; the properties of organic and inorganic substances. For example, single wall nanotubes (SWNTs) emission bands are narrow (~ 20nm) and each band corresponds to unique species (chiralities). With IMA, it is possible to separate these species with single SWNT spatial resolution on surfaces or in live cells. Biologists will love its attenuated tissue absorbance, its higher depth of penetration and its limited autofluorescence for their non-destructive analysis.

» Datasheet

IMA™ OPENS THE DOOR TO:

  • Perform complex material analyses like solar cell characterization and semiconductor quality control (e.g. perovskite, GaAs, SiC, CIS, CIGS, etc.).
  • Study IR markers in complex environments including live cells and tissue. Take for instance the spectral heterogeneity of IR fluorophores emitting in the second biological window.
  • Retrieve dark-field images and obtain a contrast of transparent and unstained samples such as polymers, crystals or live cells.

KEY CHARACTERISTICS:

  • Fast global mapping (non-scanning)
  • High spatial (<1µm) and spectral (4nm) resolution
  • Complete system (source, microscope, camera, filter, software)
  • Non-destructive analysis
  • Customisation available
  • Sensitive from 400 to 1200nm in the visible and from 900 up to 1620nm in the SWIR range

IMA™ Hyperspectral Microscopy in Action

This video shows how spectrally and spatially resolved PL and EL maps created using Photon etc's IMA can help identify defects, losses, and uniformity in advanced materials. A hyperspectral photoluminescence demonstration is performed on large grain perovskite crystals.

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Applications

PHOTOVOLTAICS

IMA™ provides spectrally and spatially resolved photoluminescence and electroluminescence images. It was successfully used to investigate spatial distribution of optoelectronic properties of CIS, CIGS, GaAs and perovskite solar cells.

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DEFECTS ON SiC

IMA™ allows a rapid and accurate identification of the class of defects that contributes to the green emission in 4H-SiC.

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MULTIPLEXING OF 17 SWCNTs

IMA™ can identify and map the distribution of 17 different species (chiralities) of carbon nanotubes. With IR hyperspectral microscopy, it is possible to separate these species, with single SWNT spatial resolution, on surfaces, in live cells (in vivo) and in vitro.

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THIN FILMS OF GaP

Spectral dependence of the Faraday magneto-optical effect with temperature dependence measurements were performed on a semiconductor 2µm epilayer (GaP) grown with embedded metallic ferromagnetic nanoclusters (MnP).

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OPTICAL CHEMOSENSOR

IMA™ provided rapid quantitative fluorescence imaging from DNA polyfluorophores. Those polyfluorophores were used as optical chemosensors for the detection of complex mixtures of hydrocarbons in contaminated soil.

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NEURONAL IMAGING

Cellular and tissue imaging are limited by the number of label, or stains, that one could use to image and study many tissue type or molecular species simultaneously. Photon etc's technology can remove these limits by using novel narrow band labels and its hyperspectral imager.

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NANOPARTICLES IN CANCER CELLS

When Photon etc's hyperspectral imager is combined with a highly effective dark field condenser, it is possible to generate high-contrast images of biological samples such as cancer cells.

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